Cow IgG Isotype Control
Quick Overview for Cow IgG Isotype Control (ABIN964172)
Target
Fragment
Application
Isotype
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Host
- Cow
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Supplier Product No.
- 001-0103
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Supplier
- Rockland
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Purpose
- Bovine IgG Fc Fragment
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(c). No reaction was observed against anti-Bovine IgG F(ab')2 or anti-Papain.
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Characteristics
- Bovine IgG F(c) Agarose Conjugate, Bovine IgG Fc Agarose, Bovine Fc fragment Agarose conjugate
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Purification
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.
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Sterility
- Sterile filtered
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-
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Application Notes
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Application Note: Bovine IgG F(c) has been tested in SDS-Page and Dot Blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- IgG
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Target Type
- Antibody
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Background
- Background: Bovine IgG F(c) Fragment Agarose Conjugated product is made from IgG, secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins followed by agarose conjugation.
Target
-